The interior of the tube wall is coated with either EDTA
K2 or EDTA K3. The tube is also available with an 8% liquid
EDTA solution. The EDTA binds calcium ions thus blocking the
coagulation cascade. VACUETTE® EDTA tubes can be used
in direct sampling analysers without actually being opened.
Erythrocytes, leucocytes and thrombocytes are stable in EDTA
anticoagulated blood for up to 24 hours. Blood smearing should
be done within 3 hours after blood collection. VACUETTE®
EDTA tubes are used for testing whole blood in the clinical
VACUETTE® EDTA K2 tubes are used for testing whole blood
in molecular diagnostics. VACUETTE® EDTA K2/gel tubes
are used for testing plasma in molecular diagnostics and viral
load detection. HIV and HCV are stable for up to 72 hours
in a non-centrifuged sample at room temperature (20–25°C).
However centrifugation for VACUETTE® EDTA K2/gel tubes
should be done within 6 hours after blood collection for best
results. Mid- term storage (up to 2 weeks) in primary tubes
is recommended at –20°C. For long-term storage (over 2
weeks) at –70°C or at lower temperatures please store
aliquots in cryo vials.